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ABSTRACT:The guinea pigs were immunized by the Brucella vaccine through intradermal injections and the skin scratch respectively ,and then the immune effects of the two ways were evaluated .Serum samples were collected one month after the last injection and detected for the total IgG titer by interval ELISA .Cell‐mediated immune was evaluated by late‐onset hyper‐sensitivity .The guinea pigs were challenged with Brucella melitensis M5 ,and then were killed to isolated M5 from spleen of each guinea pig to compare the protective effects of two methods of immunization .The ELISA results showed that both of the two methods of immunization could induce strong humoral immune response ,and DTH response to Br‐PPD antigen were 100%in both methods .No significant difference in the immune protective effect of two methods was detected .Results of humoral im‐munity ,cellular immunity and protective effect showed the same effect by intradermal injections and skin scratches .
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OBJECTIVE To isolate Acinetobacter bacteriophages and analyze their lysis spectrum.METHODS A total of 78 Acinetobacter strains were isolated from the sewage of different sources whose lysis spectrum was determined by method of single layer plates.RESULTS From them only two kinds of Acinetobacter bacteriophages AB46 and AB54 with different lysis spectrum were isolated from 2 strains.The former had a lysis spectrum of 46(1.28%)and the latter had a lysis spectrum of 54,57,59,60,62,69,70 and 78(10.27%).CONCLUSIONS Acinetobacter bacteriophages are isolated and their lysis spectrums are obtained.The lysis spectrums are narrow which need to improve their lysis ranges but they can infect their hosts virulently and form transparent plaques.